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human trf3 cdna  (OriGene)


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    OriGene human trf3 cdna
    Fig. 1 The <t>TRF3</t> expression level is enhanced during ME differentiation of hESCs. a Microscope images for the morphology of ME differentiation. Scale bar = 200 μm. b The heat map of the expression pattern of early germ layer genes during the ME differentiation of hESCs based on the microarray analysis. The expression values in log2 scale were calculated and presented on the heat map with red representing highly abundant transcripts and green representing poorly abundant transcripts. n = 3 each. c qRT-PCR analysis of TBP, TRF2, and TRF3. Data are presented as mean ± SEM. n = 3 each. *p < 0.05, **p < 0.01 compared with the corresponding undifferentiated values
    Human Trf3 Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+trf3+cdna/TBP2+(TBPL2)+(NM_199047)+Human+Tagged+ORF+Clone/pm32448362-71-7-12
    Average 90 stars, based on 2 article reviews
    human trf3 cdna - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes."

    Article Title: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes.

    Journal: Stem cell research & therapy

    doi: 10.1186/s13287-020-01711-w

    Fig. 1 The TRF3 expression level is enhanced during ME differentiation of hESCs. a Microscope images for the morphology of ME differentiation. Scale bar = 200 μm. b The heat map of the expression pattern of early germ layer genes during the ME differentiation of hESCs based on the microarray analysis. The expression values in log2 scale were calculated and presented on the heat map with red representing highly abundant transcripts and green representing poorly abundant transcripts. n = 3 each. c qRT-PCR analysis of TBP, TRF2, and TRF3. Data are presented as mean ± SEM. n = 3 each. *p < 0.05, **p < 0.01 compared with the corresponding undifferentiated values
    Figure Legend Snippet: Fig. 1 The TRF3 expression level is enhanced during ME differentiation of hESCs. a Microscope images for the morphology of ME differentiation. Scale bar = 200 μm. b The heat map of the expression pattern of early germ layer genes during the ME differentiation of hESCs based on the microarray analysis. The expression values in log2 scale were calculated and presented on the heat map with red representing highly abundant transcripts and green representing poorly abundant transcripts. n = 3 each. c qRT-PCR analysis of TBP, TRF2, and TRF3. Data are presented as mean ± SEM. n = 3 each. *p < 0.05, **p < 0.01 compared with the corresponding undifferentiated values

    Techniques Used: Expressing, Microscopy, Microarray, Quantitative RT-PCR

    Fig. 3 The expression levels of pluripotent markers are comparable among the undifferentiated TRF3+/+, TRF3−/−-1, and TRF3−/−-2 hESCs. a qRT- PCR analysis of pluripotency markers OCT4 and NANOG. n = 3. b Flow cytometry analysis of OCT4 and SSEA4. c Immunocytochemical staining analysis of OCT4 and SSEA4. Similar results were obtained from three independent experiments. Scale bar = 50 μm. d Flow cytometry analysis of cell cycle in undifferentiated hESCs. n = 3. e Representative analysis of clones positive for alkaline phosphatase staining. Similar results were obtained from three independent experiments. Scale bar = 500 μm. Data are presented as mean ± SEM
    Figure Legend Snippet: Fig. 3 The expression levels of pluripotent markers are comparable among the undifferentiated TRF3+/+, TRF3−/−-1, and TRF3−/−-2 hESCs. a qRT- PCR analysis of pluripotency markers OCT4 and NANOG. n = 3. b Flow cytometry analysis of OCT4 and SSEA4. c Immunocytochemical staining analysis of OCT4 and SSEA4. Similar results were obtained from three independent experiments. Scale bar = 50 μm. d Flow cytometry analysis of cell cycle in undifferentiated hESCs. n = 3. e Representative analysis of clones positive for alkaline phosphatase staining. Similar results were obtained from three independent experiments. Scale bar = 500 μm. Data are presented as mean ± SEM

    Techniques Used: Expressing, Quantitative RT-PCR, Flow Cytometry, Staining, Clone Assay

    Related Articles

    Clone Assay:

    Article Title: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes
    Article Snippet: .. The human TRF3 cDNA (RC211988, NM_199047, OriGene, USA) was cloned into pCDH-EF1-3×Flag-MCS-T2A-Puro, modified from pCDH-EF1-MCS-T2A-Puro (System Biosciences, CA, USA), and named as pCDH-EF1-3×Flag-TRF3-T2A-Puro. ..

    Article Title: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes.
    Article Snippet: .. Reintroduction of TRF3 into TRF3−/− hESCs The human TRF3 cDNA (RC211988, NM_199047, OriGene, USA) was cloned into pCDH-EF1-3×Flag-MCST2A-Puro, modified from pCDH-EF1-MCS-T2A-Puro (System Biosciences, CA, USA), and named as pCDHEF1-3×Flag-TRF3-T2A-Puro. ..

    Modification:

    Article Title: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes
    Article Snippet: .. The human TRF3 cDNA (RC211988, NM_199047, OriGene, USA) was cloned into pCDH-EF1-3×Flag-MCS-T2A-Puro, modified from pCDH-EF1-MCS-T2A-Puro (System Biosciences, CA, USA), and named as pCDH-EF1-3×Flag-TRF3-T2A-Puro. ..

    Article Title: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes.
    Article Snippet: .. Reintroduction of TRF3 into TRF3−/− hESCs The human TRF3 cDNA (RC211988, NM_199047, OriGene, USA) was cloned into pCDH-EF1-3×Flag-MCST2A-Puro, modified from pCDH-EF1-MCS-T2A-Puro (System Biosciences, CA, USA), and named as pCDHEF1-3×Flag-TRF3-T2A-Puro. ..



    Similar Products

    human trf3 cdna  (OriGene)
    90
    OriGene human trf3 cdna
    Fig. 1 The <t>TRF3</t> expression level is enhanced during ME differentiation of hESCs. a Microscope images for the morphology of ME differentiation. Scale bar = 200 μm. b The heat map of the expression pattern of early germ layer genes during the ME differentiation of hESCs based on the microarray analysis. The expression values in log2 scale were calculated and presented on the heat map with red representing highly abundant transcripts and green representing poorly abundant transcripts. n = 3 each. c qRT-PCR analysis of TBP, TRF2, and TRF3. Data are presented as mean ± SEM. n = 3 each. *p < 0.05, **p < 0.01 compared with the corresponding undifferentiated values
    Human Trf3 Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+trf3+cdna/TBP2+(TBPL2)+(NM_199047)+Human+Tagged+ORF+Clone/pm32448362-71-7-12
    Average 90 stars, based on 1 article reviews
    human trf3 cdna - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 1 The TRF3 expression level is enhanced during ME differentiation of hESCs. a Microscope images for the morphology of ME differentiation. Scale bar = 200 μm. b The heat map of the expression pattern of early germ layer genes during the ME differentiation of hESCs based on the microarray analysis. The expression values in log2 scale were calculated and presented on the heat map with red representing highly abundant transcripts and green representing poorly abundant transcripts. n = 3 each. c qRT-PCR analysis of TBP, TRF2, and TRF3. Data are presented as mean ± SEM. n = 3 each. *p < 0.05, **p < 0.01 compared with the corresponding undifferentiated values

    Journal: Stem cell research & therapy

    Article Title: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes.

    doi: 10.1186/s13287-020-01711-w

    Figure Lengend Snippet: Fig. 1 The TRF3 expression level is enhanced during ME differentiation of hESCs. a Microscope images for the morphology of ME differentiation. Scale bar = 200 μm. b The heat map of the expression pattern of early germ layer genes during the ME differentiation of hESCs based on the microarray analysis. The expression values in log2 scale were calculated and presented on the heat map with red representing highly abundant transcripts and green representing poorly abundant transcripts. n = 3 each. c qRT-PCR analysis of TBP, TRF2, and TRF3. Data are presented as mean ± SEM. n = 3 each. *p < 0.05, **p < 0.01 compared with the corresponding undifferentiated values

    Article Snippet: Reintroduction of TRF3 into TRF3−/− hESCs The human TRF3 cDNA (RC211988, NM_199047, OriGene, USA) was cloned into pCDH-EF1-3×Flag-MCST2A-Puro, modified from pCDH-EF1-MCS-T2A-Puro (System Biosciences, CA, USA), and named as pCDHEF1-3×Flag-TRF3-T2A-Puro.

    Techniques: Expressing, Microscopy, Microarray, Quantitative RT-PCR

    Fig. 3 The expression levels of pluripotent markers are comparable among the undifferentiated TRF3+/+, TRF3−/−-1, and TRF3−/−-2 hESCs. a qRT- PCR analysis of pluripotency markers OCT4 and NANOG. n = 3. b Flow cytometry analysis of OCT4 and SSEA4. c Immunocytochemical staining analysis of OCT4 and SSEA4. Similar results were obtained from three independent experiments. Scale bar = 50 μm. d Flow cytometry analysis of cell cycle in undifferentiated hESCs. n = 3. e Representative analysis of clones positive for alkaline phosphatase staining. Similar results were obtained from three independent experiments. Scale bar = 500 μm. Data are presented as mean ± SEM

    Journal: Stem cell research & therapy

    Article Title: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes.

    doi: 10.1186/s13287-020-01711-w

    Figure Lengend Snippet: Fig. 3 The expression levels of pluripotent markers are comparable among the undifferentiated TRF3+/+, TRF3−/−-1, and TRF3−/−-2 hESCs. a qRT- PCR analysis of pluripotency markers OCT4 and NANOG. n = 3. b Flow cytometry analysis of OCT4 and SSEA4. c Immunocytochemical staining analysis of OCT4 and SSEA4. Similar results were obtained from three independent experiments. Scale bar = 50 μm. d Flow cytometry analysis of cell cycle in undifferentiated hESCs. n = 3. e Representative analysis of clones positive for alkaline phosphatase staining. Similar results were obtained from three independent experiments. Scale bar = 500 μm. Data are presented as mean ± SEM

    Article Snippet: Reintroduction of TRF3 into TRF3−/− hESCs The human TRF3 cDNA (RC211988, NM_199047, OriGene, USA) was cloned into pCDH-EF1-3×Flag-MCST2A-Puro, modified from pCDH-EF1-MCS-T2A-Puro (System Biosciences, CA, USA), and named as pCDHEF1-3×Flag-TRF3-T2A-Puro.

    Techniques: Expressing, Quantitative RT-PCR, Flow Cytometry, Staining, Clone Assay